Fig. 1
From: Molecular dissection of a hyper-aggressive CBFB-MYH11/FLT3-ITD–positive acute myeloid leukemia
Workflow of the case molecular investigation. In order to apply a Trio analysis, peripheral blood samples from both the patient (proband, F) and the parents (P1, P2) were collected. After isolation, mononuclear cells were lysed, then DNA was extracted and purified to perform Whole Exome Sequencing. Resulting data has been analysed exploiting Illumina tools and other software, coupled with custom R scripts. This allowed the selection of relevant anomalies, which were then manually screened