Fig. 7

Senolytic reduces miRNA-326-3p containing LEVs secretion from senescent EAT and prevents onset of mitochondrial dysfunction. A Relative expression level of miRNA-326-3p in EAT-LEVs collected from vehicle or senolytic treated STZ-mice (n = 3 per group). B Expression level of miRNA-326-3p in NMVMs treated with STZ-seno- or STZ-veh-EAT LEVs (n = 3 per group). C Western blotting analysis of Rictor, p-AKT, AKT and Gapdh in STZ-seno- or STZ-veh-EAT LEVs treated NMVMs (n = 3 per group). NMVMs treated with STZ-seno- or STZ-veh-EAT LEVs and D contractile function (n = 5 per group), E mitochondrial membrane potential (TMRM) and mitochondrial superoxide (MitoSox) (n = 5 per group), F mitochondrial respiration (n = 8–9 per group) were evaluated. Data are presented as the mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared to Veh group