Fig. 1

Removal of epididymal adipose tissue alleviates diastolic dysfunction in STZ mice. A Representative images of senescence associated beta-galactosidase (SA-β-gal) staining of EAT. B Detection of SA-β-gal positive cells from Con- or STZ-EAT (n = 3 per group). C Relative gene expression of senescence associated genes in EAT from Con- or STZ-mice (n = 3 per group). D Experimental design of EAT removal surgery. E Representative of doppler and tissue doppler echocardiography and evaluation of diastolic function (n = 6 per group). F Sarcomere length tracing of isolated murine cardiomyocytes using an Ionoptix HTS system. Evaluation of G myocardial contraction, H fraction shortening and I calcium handling in Langendorff-isolated adult mouse ventricular myocytes (AMVMs) (n = 5 per group). J Representative micrographs of heart tissue sections examined by transmission electron micrographs. Data are presented as the mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared to Con group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 compared to STZ + EATr group