Fig. 8

Stress-induced CEBPB is the upstream transcription factor of TRIM2. A The Venn diagram shows the overlap of the upstream transcription factor of TRIM2 predicted by PROMO (a) and the gene regulated by EPI detected by transcriptome sequencing (b). B–D Immunoblot and qPCR analysis indicated that the expression of CEBPB is regulated by EPI. E Immunoblot analysis indicated that the expression of TRIM2 is regulated by CEBPB. F–H ChIP assays with CEBPB antibody or IgG were performed to verify binding between CEBPB and the TRIM2 promoter in 293 T cells. I-J Transwell assays revealed that CEBPB promotes migration in CRC cells. K Colony formation assays revealed that CEBPB promotes proliferation in CRC cells. L-M Western blot analysis indicated that inhibiting CEBPB expression reverses the EPI-induced promotion of EMT and CSCs in CRC cells. *P < 0.05, **P < 0.01, ***P < 0.001