Fig. 7

Stress-induced epinephrine promotes the EMT and transformation of colon cancer cells into cancer stem-like cells through the TRIM2/p53 axis. A–C Western blot analysis indicated that inhibiting TRIM2 expression reverses the EPI-induced promotion of EMT and CSCs in CRC cells. D–F Mammosphere culture analysis revealed that inhibiting TRIM2 expression reverses the effect of EPI on the promotion of CSCs in CRC cells (scale bar: 100 μm). G-H Transwell assays revealed that inhibiting TRIM2 expression reverses the effect of EPI on the promotion of migration in CRC cells. (I-J) Colony formation assays revealed that inhibiting the expression of TRIM2 reverses the effect of EPI on the promotion of proliferation in CRC cells. *P < 0.05, **P < 0.01, ***P < 0.001