Fig. 5

JAML overexpression promotes LUAD cell proliferation in vivo. a, b Knockdown or overexpression efficiency of JAML was confirmed in A549 (a) or PC9 cells (b) after stable transfection of lentivirus by Western blotting. The internal reference gene was β-actin. c, d Representative images of tumors formed by A549 (c) or PC9 cells (d) 5 weeks after subcutaneous injection, quantitative analysis n = 6, unpaired t test, *P < 0.05, ***P < 0.001, compared with the ShNC or vector groups. e, f Representative images of IHC staining for JAML in xenograft tumors caused by A549 (e) or PC9 cells (f). g, h Representative images of IHC staining for Ki67 in xenograft tumors caused by A549 (g) and PC9 cells (h)