Fig. 2

MEG3 upregulates miR-493-5p to strengthen the sensitivity of Molm13 cells to AraC. A, A volcano map of differentially expressed miRNAs in AML-related miRNA expression dataset GSE62137 (3 control samples and 15 tumor samples). Red represents significantly upregulated genes, and green represents significantly downregulated genes. B, Correlation analysis between miR-493 expression and MEG3 expression in AML samples included in TCGA. The top represents Pearson’s correlation coefficient and correlation p value. C, miR-493-5p expression in bone marrow samples of AML patients (n = 35) and healthy individuals (n = 35) determined by RT-qPCR. D, Pearson correlation analysis of MEG3 expression and miR-493-5p expression in bone marrow samples of AML patients (n = 35). E, miR-493-5p expression in HS-5, HL-60, Molm13, HL-60 R, and Molm13 R cells measured by RT-qPCR. Molm13 cells were treated with AraC (2 μM) + miR-493-5p inhibitor and Molm13 R cells were treated with AraC (2 μM) + miR-493-5p mimic. F, miR-493-5p expression in Molm13 and Molm13 R cells determined using RT-qPCR. G, CCK-8 was used to detect the viability of Molm13 and Molm13 R cells. H, Flow cytometry was used to detect apoptosis of Molm13 and Molm13 R cells. I, Expression of Bcl-2, Bax, and cleaved caspase-3 proteins in Molm13 and Molm13 R cells determined using Western blot analysis. Molm13 cells were treated with AraC (2 μM) + sh-MEG3 + miR-493-5p mimic and Molm13 R cells were treated with AraC (2 μM) + oe-MEG3 + miR-493-5p inhibitor. J, miR-493-5p expression in Molm13 and Molm13 R cells determined by RT-qPCR. K, CCK-8 assay was used to measure the viability of Molm13 and Molm13 R cells. L, Flow cytometry of apoptosis of Molm13 and Molm13 R cells. M, Bcl-2, Bax, and cleaved caspase-3 protein expression in Molm13 and Molm13 R cells determined by Western blot analysis. * p < 0.05. Cell experiments were performed three times independently