Skip to main content

Table 2 cffDNA enrichment efficiency

From: A dPCR-NIPT assay for detections of trisomies 21, 18 and 13 in a single-tube reaction-could it replace serum biochemical tests as a primary maternal plasma screening tool?

SMP#

cfDNA Extraction Stage

cffDNA Enrichment Stage

cfDNA (Copies)

cffDNA (Copies)

cffDNA (%)

cfDNA (Copies)

cffDNA (Copies)

cffDNA (%)

Enrichment (X)

1

351

14

3.90

190

15

8.05

2.07

2

579

33

5.70

329

37

11.32

1.99

3

462

26

5.57

283

29

10.25

1.84

4

372

21

5.52

218

24

11.12

2.01

5

407

21

5.20

239

25

10.36

1.99

6

434

41

9.45

286

47

16.35

1.73

7

359

35

9.75

222

38

17.12

1.76

8

564

33

5.76

325

39

11.92

2.07

9

467

23

4.98

247

26

10.52

2.11

10

478

23

4.81

219

26

11.64

2.42

  1. The copy numbers for cfDNA and cffDNA were shown as copies/reaction and directly measured with the dPCR assay without pre-amplification. X, indicating times of enrichment