Fig. 2
Inhibitory T cell expansion induced induction failure of cytotoxic CD8 T cells. a Lymphocytes co-culture with autologous tumor-cells for 2 weeks were rested without tumor cells for 2 days. Subsequently, lymphocytes (1 × 105/well) were stimulated by autologous tumor-cells (1 × 105/well) for 24 h and IFNγ secretions were detected by ELISPOT assay. Only lymphocytes samples were negative control. Experiments were conducted in duplicate. b Evaluation of KRAS mutation status on codon G12/G13 by ddPCR by using gDNA of tumor tissues and lymph nodes of C177. c Lymphocytes were stained with anti-TCRαβ, CD4, CD8α, TIM-3, PD-1, CTLA4 and LAG3 antibodies. TIM-3, PD-1, CTLA4 and LAG3 expression on CD4 or CD8 T cells were evaluated by flow cytometry respectively. The number indicates the proportion and mean fluorescence intensity (MFI) of each population in live cells