Fig. 1

5FU treatment of HT29 cells cultured in 2D, PDS and Matrigel. A Percentage metabolic activity of 2D-cultured HT29 cells (n = 12) treated with 5FU relative to untreated cells. Mean ± SEM, *p < 0.05, **p < 0.01, one-way ANOVA with Dunnett’s post hoc test. B LDH levels of 5FU-treated HT29 cells cultured in 2D (n = 9), PDS (n = 9) and Matrigel (n = 5) relative to respective untreated controls. Mean ± SEM, *p < 0.05, **p < 0.01, one-way ANOVA with Dunnett’s post hoc test. C Percentage RNA yield of 5FU-treated HT29 cells cultured in 2D (n = 9), PDS (n = 9) and Matrigel (n = 5) relative to respective untreated controls. Mean ± SEM, *p < 0.05, **p < 0.01, one-way ANOVA with Dunnett’s post hoc test