Fig. 8
hMSC-EVs enhanced OC cell sensitivity to chemotherapy drugs by transferring miR-18a-5p. CAOV3 drug-resistant cell lines were constructed. A The cisplatin sensitivity of resistant cells and parental cells was compared via MTT assay; B The IC50 value of drug-resistant cells was assessed; C The cisplatin sensitivity of cells was compared using colony formation assay; D Schematic diagram of tumorigenesis test in mice; E, F RT-qPCR was used to detect the expression of miR-18a-5p and NACC1 mRNA; G WB was employed to measure the levels of p-AKT, AKT, p-mTOR, and mTOR in cells; H Tumor volume of nude mice was detected; I, J Tumors in nude mice were separated, photographed and weighed; K HE staining was used to detect tumor nodules in lung tissues of nude mice. Independent test was repeated 3 times. Data were presented as mean ± SD. One-way ANOVA was used for comparisons between groups and Tukey's multiple comparisons test was adopted for the post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001