Fig. 5

Dysregulated autophagy in Hspa4-KO muscles. A and B Representative Western blots A and densitometry analyses B of the expression of HSPA4, LC3-I, LC3-II and p62 proteins in 4-month-old tibialis anterior. C Immunofluorescent staining of frozen sections of tibialis anterior for LC3 (green) and nuclei (blue) at 4 months of age. D Average chymotrypsin activity in 4-month-old tibialis anterior muscles. E Real time PCR analyses of the atrogenes in tibialis anterior muscles from 4-month-old mice. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 vs. WT controls, two-tailed unpaired Student's t-test. Numbers within columns indicate mice