Fig. 4

The GO and pathway enrichment, and protein to protein (PPI) network analysis of VCP-interacting proteins. A The Venn diagram showed 79 VCP-interacting proteins obtained by overlapping the candidates of CoIP/MS and the profile of VCP co-expressed genes in the HCC cohort from TCGA database. B–D The GO enriched terms of top 20 biological processes, cell components, and molecular function, respectively. E, F The enriched pathways through KEGG and Reactome database, separately. G The PPI network of 79 VCP-interacting proteins was analyzed in STRING database and further visualized by Cytoscape software (version 3.8.2). A total of 15 Hub genes were determined by CytoHubba plug-in. The colors of nodes from red to yellow indicated the overall combined scores of each molecule interacting with others from high to low. GO gene ontology, KEGG Kyoto Encyclopedia of Genes and Genomes