Table 1 Gene-specific reference primer sequences utilized for qRT-PCR analysis
Gene | Sequence |
|---|---|
GAPDH | F: TCAACAGCGACACCCACTC |
R: GCTGTAGCCAAATTCGTTGTC | |
ALP | F: CCAAAGGCTTCTTCTTGCTG |
R: CCACCAAATGTGAAGACGTG | |
RUNX2 | F: TCGCCAGGCTTCATAGCAAA |
R: GGCCTTGGGTAAGGCAGATT | |
DSPP | F: ATATTGAGGGCTGGAATGGGGA |
R: TTTGTGGCTCCAGCATTGTCA | |
OSX | F: CCTCCTCAGCTCACCTTCTC |
R: GTTGGGAGCCCAAATAGAAA | |
LMBR1 | F: GCGGGAGTCCACGATATGTTT |
R: GCTGACACTGCGAGAGTGAA | |
miR-758-5p | F: GCGGATGGTTGACCAGAGA |
R: AGTGCAGGGTCCGAGGTATT | |
U6 | F: CTCGCTTCGGCAGCACA |
R: AACGCTTCACGAATTTGCGT |