Fig. 4

Validation of hsa_circ_0000497 and hsa_circ_0000918 differentially expressed in ASC. a Generation of hsa_circ_0000497 is produced 5–13 exons of its host genes SLAIN1 and is located at chr13:78,293,666–78,327,493. b Generation of hsa_circ_0000918 is produced 16–18 exons of its host genes GMIP and is located at chr19:19,750,893–19,751,380. c Back_splicing site in the qRT-PCR product of hsa_circ_0000497 by sanger sequencing. d Back_splicing site in the qRT-PCR product of hsa_circ_0000918 by sanger sequencing. e qRT-PCR analysis of the expression of circ_0000497 in 8 sets of paired samples, including primary tumor lesions (T), tumor cells from ascites (ASC) and metastatic lesions (M) in EOC patients. The expression of hsa_circ_0000497 was similar in T, ASC, and M. f qRT-PCR analysis of the expression of circ_0000918 in 8 sets of paired samples, including T, ASC and M in EOC patients. The expression level of hsa_circ_0000918 was significantly elevated in ASC than in T and M