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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: CDK4/6 inhibitors sensitize gammaherpesvirus-infected tumor cells to T-cell killing by enhancing expression of immune surface molecules

Fig. 2

CDK4/6 inhibitors increase MHC-I surface expression on KSHV+ cells and EBV+ cells. Cells were treated either for 48 h (JSC-1 (a) and BCBL-1 (b)) or 72 h (Akata (c), and Raji (d)) with 1 μM Abe (a–d), 1 μM Pal, 5 μM Rib (g–h) or RPMI medium control. PEL cells (a, b) were then treated with sodium butyrate (0.3 mM) or RPMI medium control for another 24 h. The cells were then analyzed by flow cytometry for surface MHC-I expression. Endothelial cells (e and f) were treated for 4 days with 0.1 μM Abe, 0.5 μM Abe, or EGM-2 medium control and similarly analyzed for MHC-I expression. Results shown are one representative histograms of 3 separate experiments in af. Shown in g and h are the mean fold changes in median fluorescent intensity (MFI) and standard deviations from 3 experiments, as compared to the MHC-I in untreated control cells. Comparisons were performed using the unpaired two-tailed t-test. Asterisks indicate p values: *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant

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