Fig. 6

ANGPT2 downregulation suppressed CAV1 phosphorylation. A Immunoblot of P-CAV1 and CAV after transfection with shANGPT2 is shown in the figure. B Densitometric analysis ratio of P-CAV1/GAPDH (n ≥ 3). ^**P < 0.01 vs. NG + scra, ^#P < 0.05 vs. NG + scra, ^&P < 0.05 vs. HG + scra. C HK-2 was transfected with shANGPT2, the immunoblot of TIE2 and Src. D Densitometric analysis of TIE2 and Src (n ≥ 3). No statistical significance was observed. E Densitometric analysis of Src (n ≥ 3). No statistical significance was observed. F Immunoblot of P-CAV1 and ANGPT2 after transfection with siCAV1. G. Densitometric analysis ratio of P-CAV1/GAPDH (n ≥ 3). ^**P < 0.01 vs. NG + NC, ^##P < 0.01 vs. NG + NC, ^&&P < 0.01 vs. HG + NC. H Densitometric analysis of ANGPT2/GAPDH (n ≥ 3). ^**P < 0.01 vs. NG + NC, ns vs. NG + NC, HG + NC. HG: high glucose; NC: normal control; NG: normal glucose