Fig. 2

IL-32 promotes the occurrence of AD. A RT-qPCR was used to detect the expression of IL-4, IL-5, IL-6, IL-13 and TNF-α in HaCaT cell line (* p < 0.05 vs. control). B, ELISA detection of IL-4, IL-5, IL-6, IL-13 and TNF-α in HaCaT cell line (* p < 0.05 vs. control). C Weight of lymph nodes before and after PA treatment (n = 10, * p < 0.05). D Clinical scores of dorsal skin. E Thickness of epidermis. F HE staining for IL-32 in epidermal cells of mouse model before and after PA treatment and quantitative results (50 μm, n = 10, * p < 0.05). G Immunohistochemical staining for IL-32 of epidermal cells in mouse model before and after PA treatment and quantitative results (50 μm, n = 10, * p < 0.05). Cell experiment was repeated for three times