Fig. 5

MiR-874-3p targets CPEB4 in EC cells. a Venn diagram suggested that 2 genes might be targets of miR-874-3p. b, c The relative RNA and protein levels of CRCP and CPEB4 were determined by qRT-PCR and Western blot analyses, respectively, after transfection with miR-874-3p mimic and inhibitor in Ishikawa cells and RL952 cells. d The relative RNA levels of CPEB4 in EC tissues and normal tissues were determined by qRT-PCR. e The luciferase reporter assay functionally validated the interaction between miR-874-3p and CPEB4 in Ishikawa cells and RL952 cells. f The CPEB4 expression in Ishikawa cells and RL952 cells after transfection of shRNA was determined by qRT-PCR analysis. g The impacts of CPEB4 knockdown on the migration and invasion of Ishikawa cells and RL952 cells were examined by using Transwell assays. h The expression of EMT-related proteins was detected by Western blot analysis. Scale bar: 100 μm, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001