Fig. 4

CircESRP1 acts as a sponge for miR-874-3p. a Venn diagram suggested that 3 miRNAs might be targets of circESRP1 sponge action. b, c RNA pulldown was performed in Ishikawa cells and RL952 cells, followed by qRT-PCR to detect the enrichment of circESRP1 and related miRNAs. d Anti-AGO2 RIP was performed in Ishikawa cells and RL952 cells, followed by qRT-PCR to detect the capacity for AGO2 enrichment on circESRP1 and miR-874-3p compared to IgG. e The luciferase reporter assay functionally validated the interaction between circESRP1 and miR-874-3p in in Ishikawa cells and RL952 cells. f Transwell assays demonstrated the effects of the miR-874-3p mimic and inhibitor on Ishikawa cells and RL952 cell migration and invasion. g The relative RNA levels of miR-874-3p in EC tissues and normal tissues were determined by qRT-PCR. h The negative interaction between miR-874-3p and circESRP1. Scale bar: 100 μm, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001