Fig. 2

Knockdown of circESRP1 suppressed EMT and EC cell proliferation, migration, and invasion. a qRT-PCR analysis of circESRP1 expression in Ishikawa cells and RL952 cells after transfection of the expression shRNA. b–d CCK-8, EdU incorporation, and colony formation assay were carried out to assess the proliferation of Ishikawa cells and RL952 cells. e, f Transwell and wound healing assays (magnification, ×40) were performed to assess the migration and invasion of Ishikawa cells and RL952 cells. g EMT-related proteins (E-cadherin and Vimentin) were detected by Western blot analysis in Ishikawa cells and RL952 cells transfected with sh-circESRP1. Scale bar: 100 μm. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001