Fig. 5

Response to NAMPT inhibition in MM and THCA cell lines according to BRAF mutations. A Short-term proliferation assay comparing sensitivity to NAMPTi (FK866 and GMX1778 at 25 nM, 72 h of treatment) of MM BRAF-mutated vs. WT cell lines (upper graphs) and THCA BRAF-mutated vs. WT cell lines (lower graphs). Data represent the average and SEM of at least three biological replicates. Unpaired t test was used to calculate the statistical difference of sensitivity between BRAF WT vs. mutated cell lines. B, C Proliferation assay comparing sensitivity to NAMPTi in MEL-505 (B) and Mewo (C) infected with a CTRL or BRAF V600E construct. Data represent the average and SEM of at least three biological replicates. Significance was represented as: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001