Fig. 6

CDK6 overexpression recovers the proliferation and glycolysis of CRC cells which is restrained by the transfection of miR-500a-3p mimics. A–G SW480 cells were transfected with miR-NC, miR-500a-3p, miR-500a-3p + vector or miR-500a-3p + CDK6. A The proliferation ability of SW480 cells after transfection was measured by CCk-8 assay. B The colony formation ability of after transfection were measured by colony formation assay. Representative images (left) and quantification (right) were shown. C The cell cycle was evaluated by flow cytometry after transfection. Representative images (left) and quantification data (right) were shown. D–F The glucose uptake (D), lactate production (E), intracellular ATP (F) in SW480 cells were measured after transfection using Glucose Uptake Colorimetric Assay kit, Lactate Assay Kit and ATP Colorimetric Assay kit. G Seahorse XF 96 Extracellular Flux Analyzer was used to detect the ECAR in four groups of SW480 cells. Data were shown as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001