Fig. 2

miR-500a-3p suppress CRC growth in vitro and in vivo. A, B CCK-8 assay showed the proliferation ability after transfection of miR-NC and miR-500a-3p in SW480 and HCT-116 cells. C, D The plate colony formation ability of SW480 and HCT-116 cells after transfection with miR-NC or miR-500a-3p mimics were measured by colony formation assay. Representative images (left) and quantification (right) were shown. E, F Flow cytometry showed the cell cycle in SW480 and HCT-116 cells. The data from A to F were presented as the means ± SD (n = 3). G Nude mice (n = 7) were subcutaneously injected with SW480 cells transfected with miR-NC or miR-500a-3p. Representative tumor diagrams in different groups were shown. H, I Volume and weight of xenograft tumor were measured in the miR-500a-3p and miR-NC group. Tumor volume was analyzed every 5d using the formula: volume = (length × width2)/2. J, K The subcutaneous tumors were stained with ki67. Scale bar, 100 μm. The quantification of ki67 IHC score in miR-500a-3p and miR-NC group were shown (n = 7). *P < 0.05, **P < 0.01, ***P < 0.001