Fig. 7

Circ_0036412 propels the proliferation and suppresses cell cycle arrest of HCC cells in vitro through Hedgehog pathway. A, B CCK-8 and EdU assays assessed cell proliferation in HuH-7 cells after the transfection of si-NC, si-circ_0036412-1, si-circ_0036412-1 + pcDNA3.1 or si-circ_0036412-1 + pcDNA3.1-GLI2 (One-way ANOVA, Tukey). C Flow cytometry analysis detected cell cycle in HuH-7 cells after the transfection of si-NC, si-circ_0036412-1, si-circ_0036412-1 + pcDNA3.1 or si-circ_0036412-1 + pcDNA3.1-GLI2 (One-way ANOVA, Tukey). D, E The mRNA levels of CDC2 and CCNB1 were evaluated by qRT-PCR in HuH-7 cells after the transfection of si-NC, si-circ_0036412-1, si-circ_0036412-1 + pcDNA3.1 or si-circ_0036412-1 + pcDNA3.1-GLI2 (One-way ANOVA, Tukey). *P < 0.05, **P < 0.01