Fig. 6

Circ_0036412 stabilizes GLI2 expression by recruiting ELAVL1. A StarBase predicted the RBPs interacting with circ_0036412 and GLI2. B RNA pulldown assay followed by western blot detected the interaction of circ_0036412 with IGF2BP2 or ELAVL1 in HuH-7 cells. C RNA pulldown assay followed by western blot detected the interaction of circ_0036412 with ELAVL1 in HuH-7 and Hep 3B cells. D, E RIP assay followed by qRT-PCR assessed the interaction of ELAVL1 with circ_0036412 and GLI2 3’UTR in HuH-7 and Hep 3B cells (Student’s t test). F, G The half-lives of GLI2 and β-actin were assessed by qRT-PCR in α-amanitin-treated HuH-7 cells after the knockdown of ELAVL1 or circ_0036412 (Student’s t test). H The half-lives of GLI2 and β-actin were assessed by qRT-PCR in α-amanitin-treated HuH-7 cells after the transfection of pcDNA3.1, pcDNA3.1-circ_0036412, pcDNA3.1-circ_0036412 + si-NC or pcDNA3.1-circ_0036412 + si-ELAVL1-1 (One-way ANOVA, Tukey). **P < 0.01