Fig. 3

Circ_0036412 modulates Hedgehog signaling pathway in HCC cells. A–C The expressions of IkBα, RelA, IL-6, GSK3β, CTNNB1, C-Myc, PTCH1, GLI2 and CCND1 were detected by qRT-PCR in HuH-7 and Hep 3B cells after the transfection of si-circ_0036412-1/2 (One-way ANOVA, Dunnett). D The protein levels of nuclear GLI2, cytoplasmic GLI2 and CCND1 were detected by western blot in HuH-7 and Hep 3B cells after the transfection of si-circ_0036412-1/2. E FISH assay detected the co-localization of circ_0036412 and GLI2 in HuH-7 and Hep 3B cells. F RIP assay detected the existence of circ_0036412 and GLI2 in RISC in HuH-7 and Hep 3B cells (Student’s t test). **P < 0.01