Fig. 3

Combination treatment reduced the severity of GvHD. a Survival rate, weight loss, and clinical score after BMT in GvHD mice. Allogeneic‑transplanted animals showed a significantly increased GvHD score compared with syngeneic-transplanted animals. The clinical GvHD score was the sum of weight loss, posture, activity, fur, and skin on a scale of 0–2 (n = 5 per group and repeated three times). b Four weeks after BMT, IFN-γ and IL-17 expression in mouse CD4⁺-T cells as analyzed by flow cytometry. c Histopathology of the skin, liver, and large intestine after BMT (n = 5 per group) from one of two independent experiments. Sections were stained with hematoxylin and eosin (original magnification; liver × 40, skin and large intestine × 200; scale bar, 100 μm). Histopathology score of skin, liver, and large intestine tissues. Results are means ± SEM (*each sample versus vehicle; ^#combination versus L. acidophilus; ^Xcombination versus FK506, *^,#,Xp < 0.05, **^,##,XXp < 0.01, ***^,###,XXXp < 0.005)