Fig. 2

Up-regulated miR-200c inhibited proliferation, migration and invasion in bladder cancer cells. A Entivirus carrying miR-200c plasmid and the control plasmid were persistently co-transfected into UMUC-3 and T24 cells. Measurements by real-time RT-PCR of miR-200c confirmed our success of transduction and were obviously higher than the control group in both cell lines. B CCK-8 assays revealed cell growth differences of indicated cell lines. C Colony formation assay in T24 and UMUC-3 cells. D Measurement of in vitro cell migration by “wound-healing” assay. Representative pictures (left) and quantification (right) for same single spot in indicated cell lines. E Transwell migration assays in indicated engineered cell lines. F Transwell invasion assays in indicated engineered cell lines. Data are presented as mean ± SD from 3 independent experiments. *P < 0.05; **P < 0.01. ***P < 0.001; DAPI, 4′,6-diamidino-2-phenylindole