Fig. 3

The downstream gene of UAP1L1 was screened by IPA. A After UAP1L1 knockdown, the state of signaling pathways in prostate cancer cells was analyzed by IPA. B The interaction network of genes and pathways was assessed by IPA. C Co-IP was used to verify the interaction between UAP1L1 and CDCA8, which was indicated that UAP1L1 protein interacted with CDCA8 protein. D The expression levels of CDCA8 was upregulated in prostate cancer tissues, which was detected by immunohischemical staining. Magnification was 200 times. E The expression of CDCA8, downstream molecules of UAP1L1, and proteins related to pathways was detected by western blot. ShCtrl: prostate cancer cells infected with shRNA of control; shUAP1L1: prostate cancer cells infected with shRNA of UAP1L1. Input: whole cell lysate of C4-2 cell group; IgG: control group by immunoprecipitation with IgG; CDCA8: protein obtained by immunoprecipitation with CDCA8 antibody.