Fig. 8

CircDYRK1A inhibits the malignant phenotypes and glutamine metabolism in GC cells by upregulating the expression of FBXO4 through miR-889-3p. AGS cells were transduced with sh-FBXO4-1 or sh-FBXO4-2. A Expression of FBXO4 in AGS cells determined by RT-qPCR. AGS cells were transduced with sh-FBXO4 and/or circDYRK1A, and HGC-27 cells were transduced with oe-FBXO4 and/or sh-circDYRK1A. B Expression of FBXO4, circDYRK1A and miR-889-3p in AGS cells and HGC-27 cells determined by RT-qPCR. C Proliferation of AGS cells and HGC-27 cells detected by EdU assay. D Migration of AGS and HGC-27 cells detected by Transwell assay. E Invasion of AGS and HGC-27 cells detected by Transwell assay. F Expression of glutamine in AGS and HGC-27 cells measured using the kit. G Expression of glutamic acid in AGS and HGC-27 cells measured using the kit. H Expression of α-KG in AGS and HGC-27 cells measured using the kit. I Protein levels of GLS and GDH in AGS and HGC-27 cells measured by Western blot analysis. The cell experiment was repeated three times. * p < 0.05