Fig. 5

CircDYRK1A sponges miR-889-3p in GC cells. A Venn map of intersection of upregulated miRNA set among GC-related miRNA set through MNDR database, circDYRK1A targeted miRNA set predicted by Circinteractome database, and differential analysis of microarray dataset GSE54397. B hsa-miR-889-3p expression in STAD patients in starBase database. C Targeted binding sites of hsa_circRNA_001826 and hsa-miR-889-3p. D miR-889-3p expression in GC tissues and adjacent normal tissues determined by RT-qPCR (n = 50). E Pearson correlation analysis of the correlation between miR-889-3p expression and circDYRK1A expression in GC tissues. AGS cells were transduced with circDYRK1A overexpression vector and HGC-27 cells were transduced with sh-circDYRK1A. F miR-889-3p expression in AGS and HGC-27 cells determined by RT-qPCR. G The binding of miR-889-3p and circDYRK1A detected by dual luciferase reporter assay in 293 T cells. H The binding of miR-889-3p and circDYRK1A with AGO protein detected by RIP assay. I miR-889-3p pull down circDYRK1A detected by RNA pull down assay. The cell experiment was repeated three times. * p < 0.05 vs. adjacent normal tissues, 293 T cells treated with mimic-NC, sh-NC-treated HGC-27 cells, Vector-treated AGS cells, or AGS and HGC-27 cells treated with IgG, or biotin-NC