Fig. 3
From: IRF-2 inhibits cancer proliferation by promoting AMER-1 transcription in human gastric cancer
IRF-2 directly activated AMER-1 transcription and regulated the Wnt/β-catenin signaling pathway. A The peak information in ChIP-Seq analysis and proportion of IRF-2 binding to promoter regions. B Enrichment analysis of GO-Biological Precell with IRF-2 expression was shown. C KEGG analysis also showed that IRF-2 may affect several cancer pathways. D ChIP-Seq showed that IRF-2 may act on the promoter region of AMER-1. E Possible IRF-2 binding sites were found using the JASPAR 2020 database. F It was found that there were two predicted IRF-2 binding sites in the AMER-1 transcription start domain. G Luciferase assays for detecting luciferase activity of AMER-1-promoter-WT, AMER-1-promoter-Mut1 and AMER-1-promoter-Mut2 after IRF-2 overexpression