Fig. 4

Validation of potential roles of selected circRNAs. A circRNA-miRNA-mRNA network of the five WNT-associated CSF-circRNAs. B Quantification of five circRNAs by RT-PCR and agarose gel electrophoresis. GAPDH was used as internal control. C NGS of circRNAs after RT-PCR. D Detection of cell growth by Celigo automated imaging and cell counting platform. Triple experiments were performed. Values were represented as mean ± SD. T-test was used for comparison. *: P < 0.05