Fig. 4
Silencing WT1 expression suppressed the migration of BRAFV600E PTC cells. A, B Transwell assays showing the number of migrating IHH4 and BCPAP cells after WT1 expression was inhibited. C, D A healing wound assay indicating suppressed migration of IHH4 cells and BCPAP cells after WT1 expression was inhibited. E, F Western blot assays confirmed that silencing WT1 expression decreased the protein levels of N-cadherin, vimentin and MMP1 in IHH4 and BCPAP cells. G–J Immunofluorescence assays showing that WT1 inhibition suppressed N-cadherin and vimentin expression in IHH4 and BCPAP cells (*P < 0.05, **P < 0.01)