Fig. 3
WT1 knockdown inhibited the proliferation of BRAFV600E PTC cells. A, B Quantitative RT-PCR analysis of the WT1 expression level in the IHH4 cells and BCPAP cells treated with WT1 shRNA. C, D Western blot assay showing the knockdown efficiency of WT1 in IHH4 cells and BCPAC cells treated with lentiviral WT1 shRNA. E, F CCK-8 assay indicating the proliferation of IHH4 and BCPAC cells upon WT1 inhibition. G, H EdU assay indicating the anti-proliferative effect of WT1 shRNA treatment in IHH4 and BCPAC cells. I, J Colony formation assay showing the proliferation ability of IHH4 and BCPAC cells with WT1 knocked down. K, L The protein expression levels of PCNA and C-myc in IHH4 and BCPAP cells with WT1 knocked down, as determined by Western blot assay. M, N Changes in the protein expression levels of PCNA in IHH4 and BCPAP cells after WT1 inhibition by immunofluorescence assay (**P < 0.01, ****P < 0.0001)