Fig. 6
MAL inhibited the proliferation and promoted the apoptosis of GC cells in a mouse tumorigenesis model. MGC-803 cells with MAL knockdown or overexpression were injected subcutaneously at a dose of 107 cells per site, and xenograft tumors were dissected and removed for histopathological analysis 14 days after injection. The bar graph shows the quantitative analysis of the weight of gastric tumors (B). HE staining was performed to analyze the pathological changes in the control, MAL knockdown, and MAL-overexpressing MGC-803 cells (A). Immunohistochemistry revealed the expression of Ki-67 in control, MAL knockdown, and MAL-overexpressing MGC-803 cells (C). Immunohistochemistry revealed the expression of PCNA in control, MAL knockdown, and MAL-overexpressing MGC-803 cells (E). The TUNEL assay was used to verify cell apoptosis in control, MAL knockdown, and MAL-overexpressing xenograft tumor tissues (G). The bar graph shows the proportion of cells with positive staining for Ki-67 (D), PCNA (F) and TUNEL (H). The experiments were performed at least 3 times independently, and one representative result is shown. The results are presented as the means ± SD. *P < 0.05 and **P < 0.01. A–C, F–G, I–K and M–O, Scale bars = 100 μm