Fig. 3

The MAL protein interferes with the proliferation, metastasis and invasion of GC cells. Western blotting confirmed that transfection of MGC-803 and HGC-27 cells with the siRNA significantly downregulated the expression of the MAL protein, while transfection with the MAL expression plasmid upregulated MAL expression (A). The CCK-8 results showed that MAL knockdown increased the proliferation of MGC-803 and HGC-27 cells, while MAL overexpression inhibited the proliferation of MGC-803 and HGC-27 cells (B). MAL silencing increased the metastasis of MGC-803 cells, and MAL overexpression reduced the metastasis of MGC-803 cells (C–F). MAL knockdown increased the metastasis of HGC-27 cells, and MAL overexpression inhibited the metastasis of HGC-27 cells (G–J). MAL silencing increased the invasion of MGC-803 cells, and MAL overexpression inhibited the invasion of MGC-803 cells (K–N). MAL knockdown enhanced the metastasis of HGC-27 cells, and MAL overexpression inhibited the metastasis of HGC-27 cells (O–R). The experiments were performed at least 3 times independently, and one representative result is shown. The results are presented as the means ± SD. *P < 0.05 and **P < 0.01. C–E, G–I, K–M and O–Q, Scale bars = 100 μm