Fig. 3
From: Downregulation of NEDD4L by EGFR signaling promotes the development of lung adenocarcinoma
NEDD4L regulated the activity of the mTOR pathway in LUAD cells. A GSEA results indicated that genes in the NEDD4L low expression subgroup were enriched in the mTORC1 pathway. B Relative mRNA levels of mTORC1 pathway-related genes in samples from the TCGA_LUAD dataset. (C&D) Western blot analysis of PC9 cells stably overexpressing NEDD4L (C) or stably knocking down NEDD4L (D). E Immunofluorescence microscopy analysis of PC9 cells stably overexpressing NEDD4L or stably knocking down NEDD4L