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Table 1 The information of oligonucleotide sequences

From: Circular RNA circCSPP1 promotes the occurrence and development of colon cancer by sponging miR-431 and regulating ROCK1 and ZEB1

Gene

Sequence (5′–3′)

ROCK1 shRNA

sense: CACCGCATTTGGAGAAGTTCAATTGCGAACAATTGAACTTCTCCAAATGC

antisense: AAAAGCATTTGGAGAAGTTCAATTGTTCGCAATTGAACTTCTCCAAATG

ZEB1 shRNA

sense: CACCGAGAGAGAGAGTTTGACAAGGCGAACCTTGTCAAACTCTCTCTCTC

antisense: AAAAGAGAGAGAGAGTTTGACAAGGTTCGCCTTGTCAAACTCTCTCTCTC

circCSPP1 shRNA1

sense: CACCGCTCCAGACAATGAAACATCCCGAAGGATGTTTCATTGTCTGGAGC

antisense: AAAAGCTCCAGACAATGAAACATCCTTCGGGATGTTTCATTGTCTGGAGC

circCSPP1 shRNA2

sense: CACCGCTAATCAAGATACCTGTAGTCGAAACTACAGGTATCTTGATTAGC

antisense: AAAAGCTAATCAAGATACCTGTAGTTTCGACTACAGGTATCTTGATTAGC

shRNA control

sense: GATCCGTTCTCCGAACGTGTCACGTTTCAAGAGAACGTGACACGTTCGGAGAACTTTTTTG

antisense: AATTCAAAAAAGTTCTCCGAACGTGTCACGTTCTCTTGAAACGTGACACGTTCGGAGAACG

miR-431 mimic

UGUCUUGCAGGCCGUCAUGCA

mimic control

UUCUCCGAACGUGUCACGUTT

miR-431 inhibitor

UGCAUGACGGCCUGCAAGACA

inhibitor control

UUCUCCGAACGUGUCACGUTT

miR-324-5p mimic

CGCAUCCCCUAGGGCAUUGGUG

miR-375 mimic

UUUGUUCGUUCGGCUCGCGUGA

miR-486-3p mimic

CGGGGCAGCUCAGUACAGGAU

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Journal of Translational Medicine

ISSN: 1479-5876

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