Fig. 3

P. pentosaceus CECT 8330 increases tight junction proteins expression. A Representative experiment of ZO-1 and Occludin proteins in the colon examined by Western blot. B The relative amounts of ZO-1 and Occludin calculated by densitometry of protein bands from three independent experiments. β-Actin and GAPDH were used as loading controls, respectively. Significance was determined by ANOVA with Tukey’s analysis, *P < 0.05, **P < 0.01, ***P < 0.001