Fig. 1

Identification of heart-specific hypermethylation markers with MCTA-seq. a Heatmap of 6 identified heart-specific hypermethylation marker methylation levels and the expression levels in 9 different tissues, including the heart and WBCs. Each column represents one tissue type, and each row represents a marker. The markers (n = 6) are ranked by their methylation levels in the tissue, as calculated by their MePM values of MCTA-seq. In the heatmap, blue indicates low, white and yellow indicate intermediate and red indicates high DNA methylation values, which are shown as log₂(MePM) (left). The expression levels are shown by the log₂(z-score) (right). See the online methods for the identification of heart-specific hypermethylation markers. b–g Comparison of the representative heart-specific marker methylation levels in the plasma of MI patients (n = 20), normal plasma (n = 202), and WBCs (n = 81). MI indicates patients with acute myocardial, PN indicates normal control individuals, and WBC indicates samples of white blood cell. ****P < 0.0001. Two-tailed MWW test