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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Pan-cancer quantitation of epithelial-mesenchymal transition dynamics using parallel reaction monitoring-based targeted proteomics approach

Fig. 2

PRM-based quantitation of EMT associated proteins across a panel of cancel cell lines. Workflow illustrating the targeted proteomics-based evaluation of EMT-associated proteins in cancer cell lines. The time-scheduled Parallel reaction monitoring (PRM) assay was developed by monitoring more than 3 product ion transitions per peptide precursor. Reverse calibration using synthetic light and SIL peptides was generated to assess LOD, LOQ and linearity. SIL peptides were added to peptide digest from each cell line for normalization and quality assessment and quantitation

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Journal of Translational Medicine

ISSN: 1479-5876

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