Fig. 5

Bilirubin alters the composition of T cells in the spleen. After determining the expression of 26 surface markers on the spleen cells using mass cytometry, the T cells expressing CD3 were gated and analyzed using FlowSOM. 30 T cell clusters were identified using FlowSOM. A viSNE was used to visualize the distribution of the spleen T cells. 30 T cell clusters were identified and colored on the viSNE map. B A heatmap showing the normalized expression of 12 indicated markers in 30 T cell clusters. C Bar plots showing the frequencies of T5, T16, T6, T13, or T23 in spleen T cells obtained from ApoE^−/− mice treated with or without bilirubin. D A heatmap showing the Pearson correlation coefficients for relationships between the concentrations of total bilirubin (TBIL), LDL, HDL, TG, or TCHO in peripheral blood and the frequencies of indicated spleen T cell clusters. E Dot plots (n = 22) showing the Pearson correlation coefficients for relationships between the concentrations of LDL or TCHO in peripheral blood and the frequencies of T5, T16, or T23 clusters. Error bars represent mean ± SD. Dots represent individual samples, n (control) = 8, n (bilirubin) = 14. *P < 0.05. Differences were determined by an unpaired t-test. Correlations were determined by a Pearson test