Fig. 5
YBX1/CTPS1 axis is involved in the progression of TNBC. a, b Cell proliferation and invasion ability was assessed by CCK-8 and transwell assay after overexpression of YBX1 and downregulation of CTPS1. All data are presented as the mean ± SD of three independent experiments. c-f The correlation between YBX1 and CTPS1 mRNA expression was evaluated by Spearman’s correlation analysis based on GEO (GSE21653, GSE31448, GSE45827) and TCGA databases. g The protein expression of YBX1 in tumor and adjacent normal tissues from triple-negative breast cancer patients was detected by IHC analysis. h Representative staining images of CTPS1 and YBX1 in TNBC tumor tissues (× 200). i The correlation between YBX1 and CTPS1 protein expression was evaluated by Spearman’s correlation analysis based on IHC analysis. j Kaplan–Meier analysis of the disease-free survival with different YBX1 expression in TNBC patients. k Kaplan–Meier analysis of the disease-free survival with different YBX1 and CTPS1 expression in TNBC patients. Scale bar: 50um. ** p < 0.01, *** p < 0.001, ###p < 0.001 (YBX1 + shCtrl vs YBX + CTPS1)