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Table 5 Expansion of Treg cells

From: Process development and validation of expanded regulatory T cells for prospective applications: an example of manufacturing a personalized advanced therapy medicinal product

 

Culture day

Freshly expanded

Recovered after thawing

#01 [KD1]

#02 [KD2]

#03 [LD1]

#04 [LD2]

Total cells fold expansion

0

1.0

1.0

1.0

1.0

7

3.8

2.9

3.2

3.5

14

26.0

28.6

30.0

28.2

21

107.1

134.9

115.2

196.1

Treg cells fold expansion

0

1.0

1.0

1.0

1.0

7

Nd

2.9

3.0

4.6

14

27.9

32.0

44.8

39.0

21

124.0

162.0

190.6

271.9

Number of Treg cells (normalized over seeded cells) (× 106)

0

34.0

32.5

21.6

28.2

7

Nd

94.1

63.8

131.0

14

948.2

1041.2

965.3

1102.4

21

4211.7

5268.3

4110.2

7678.8

  1. On day 0, 7, 14, 21 expanding cells were phenotyped for Treg markers and enumerated by flow cytometry with a single platform (Trucount)