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Table 5 Expansion of Treg cells

From: Process development and validation of expanded regulatory T cells for prospective applications: an example of manufacturing a personalized advanced therapy medicinal product

  Culture day Freshly expanded Recovered after thawing
#01 [KD1] #02 [KD2] #03 [LD1] #04 [LD2]
Total cells fold expansion 0 1.0 1.0 1.0 1.0
7 3.8 2.9 3.2 3.5
14 26.0 28.6 30.0 28.2
21 107.1 134.9 115.2 196.1
Treg cells fold expansion 0 1.0 1.0 1.0 1.0
7 Nd 2.9 3.0 4.6
14 27.9 32.0 44.8 39.0
21 124.0 162.0 190.6 271.9
Number of Treg cells (normalized over seeded cells) (× 106) 0 34.0 32.5 21.6 28.2
7 Nd 94.1 63.8 131.0
14 948.2 1041.2 965.3 1102.4
21 4211.7 5268.3 4110.2 7678.8
  1. On day 0, 7, 14, 21 expanding cells were phenotyped for Treg markers and enumerated by flow cytometry with a single platform (Trucount)