Fig. 4From: Process development and validation of expanded regulatory T cells for prospective applications: an example of manufacturing a personalized advanced therapy medicinal productFunctional properties of thawed Treg from KD (dark gray) and LD (grey) patients. The suppressive function of expanded Treg was analyzed by measuring the proliferation of CFSE-stained CD8−CD25− cells primed with anti-CD3/CD28 beads in co-culture with expanded Treg cells for 5 days. Percentages of suppression are presented as mean values; bars represent standard deviations of 2 independent experimentsBack to article page