Fig. 3
Ex vivo Treg cell expansion. Isolated CD8−CD25+ cells derived from KD and LD patients were expanded in vitro in gas-permeable culture bags for 3 weeks in complete medium. A Kinetics of proliferation at a clinical scale. B Day-21 expression of discriminating markers CD127 and FoxP3; C frequency of contaminating populations. Treg expanded after thawing (n = 1, run #04 LD2, diamond shape) displayed no relevant differences as compared to freshly expanded Treg (all other symbols). D The mean achievable absolute number of cells in relevant subpopulations. Frequencies were expressed as percentage of viable cells; absolute numbers were normalized over seeded cells