Fig. 6

Effect of NQO1 overexpression on HG-induced apoptosis in HK-2 cells. A–C The protein levels of Bax, Bcl-2 and Cleaved Caspase-3 were analyzed by western blotting (n = 3). D The expression levels of Bax, Bcl-2 and Cleaved Caspase-3 were measured by immunofluorescence (scale bar, 50 μm, n = 6). D Apoptosis in HK-2 cells was determined by TUNEL assays and the percentage of TUNEL-positive cells per field (× 400) (Scale bar, 50 μm, n = 6). NG: 5.6 mM d‐glucose, M: NG + mannitol (24.4 mM), HG: 30 mM d‐glucose, HG + C: HG + control pcDNA3.1(+), HG + NQO1 O/E: HG + NQO1 pcDNA3.1(+). Values are expressed as the mean ± SD. **P < 0.01 versus NG group; ##P < 0.01 versus HG + C group