Fig. 3

Determination of the shelf-life of the cryopreserved CAR/TCR T-cell flow cytometry quality controls post-thaw (open-vial stability). The cryopreserved flow cytometry quality control cells were thawed and stored at room temperature. The cells were stained at 0 h, 2 h, 4 h and 6 h post-thaw. Flow cytometry was used to assess cell viability and the expression of surface CAR/TCR markers: protein L, CD22-Fc (Siglec-2) and CD19-Fc for CD19/CD22 bispecific CAR-T cell (A), EGFR for FGFR4 CAR T cell (B) and murine TCR-beta for KK-LC-1 TCR T-cell (C). Data are shown as mean ± SD. The upper and lower bounds (± 20% range of values measured at time of cryopreservation) are also shown in each plot. Data are representative of two independent experiments with similar results. For CD19/CD22 bispecific CAR T-cell and KK-LC-1 TCR T-cell, the study was performed in triplicates. For FGFR4 CAR T-cell, the study was performed in duplicates